产品描述

细胞名称人宫颈癌细胞；C-33A

形态特性上皮样

生长特性贴壁生长

特征特性该细胞系由AuerspergN建立，源于宫颈癌活检标本。p53+，pRB+，在273位密码子发生点突变。乳头状瘤病毒DNA、RNA阴性。

培养条件MEM-EBSS:MinimumEssentialMedium(MEMEagleswithEarle＇sBalancedSalts)10%FBS　

传代方法1:3~1:8传代；2~3天换液1次。

冻存条件基础培养基+5%DMSO+20%FBS　

Organism：Homosapiens,human

Tissue：cervix

CellType：Epithelial,Retinoblastoma

ProductFormat：frozen

Morphology：epithelial

CultureProperties：adherent

BiosafetyLevel：1

Disease：carcinoma

Age：66yearsadult

Gender：female

Ethnicity：Caucasian

Applications：Thelineexhibitedahypodiploidkaryotypeinitially,andanepithelialmorphology.

Theretinoblastomaprotein(pRB)ispresentbutabnormalinsize.

Expressionofp53iselevated,andthereisapointmutationatcodon273resultinginaArg->Cyssubstitution.

Karyologicalinstabilitywasobservedwithcontinuedpassage.

TheC-33Acelllineisoneofaseriesoflines(seealsoATCCCRL-1594andATCCCRL-1595)derivedbyN.Auerspergfromcervicalcancerbiopsies.

ThecellsarenegativeforhumanpapillomavirusDNAandRNA.

StorageConditions：liquidnitrogenvaporphase